The first game of the 2024 NCAA Tournament will feature No. 16 seeds Wagner and Howard in a game scheduled to tip off at 6:40 on Tuesday.
On Wednesday night, No. 16 seeds Montana State and Grambling State will tip at 6:40 followed by 10th-seeded Boise State and Colorado, teams that finished second and third in the Mountain West and Pac-12, respectively, in the regular season.
Wagner (16-15) finished sixth in the Northeast Conference but won the league tournament to earn its first NCAA Tournament bid since 2003.
Howard was the No. 4 seed in the Mid-Eastern Athletic Conference in the regular season but won the MEAC Tournament for the second time in three years.
Montana State is just 17-17 and finished fifth in the Big Sky, but winning that tournament has the Bobcats in the NCAA Tournament for the sixth time and third year in a row.
Grambling will be making its first NCAA Tournament appearance after winning the Southwest Athletic Conference for the first time. The 20-14 Tigers were the regular season champs of the SWAC as well.
Colorado State (24-10) finished tied for sixth in the loaded Mountain West while 23-10 Virginia was third in the ACC.
Of the eight teams in the 2024 First Four, only Boise State is a repeat visitor.